March 27, 2025

AMBOSS closes €240M financing round and expands its position as the leading co-pilot for physicians worldwide

AMBOSS

Investment raised

EUR 240m

Valuation

Sector

Region

🏆 Deal of the Week · CW 13/2025

AMBOSS raised EUR 240 million in a venture financing round, with the valuation remaining undisclosed. The round was backed by KIRKBI, Lightrock, and M&G Investments. This transaction was recognised as Deal of the Week for KW 13/2025 on Venionaire DealMatrix.

AMBOSS — key deal facts

Company AMBOSS
Founded 2012
Headquarters Berlin, Germany
Amount raised EUR 240m
Valuation Valuation Remains Undisclosed
Round Venture – Series Unknown
Investors KIRKBI, Lightrock, M&G Investments
Sectors Health Cares

Who invested in AMBOSS?

The round was co-funded by KIRKBI, the private investment arm of the Kirk Kristiansen family and owner of the LEGO brand, alongside impact-focused growth investor Lightrock and global asset manager M&G Investments. The investor mix is notable for combining a family-office-backed strategic vehicle, an impact-growth platform, and a large institutional asset manager — a coalition that signals conviction in AMBOSS's long-term commercial and social relevance. No single lead investor has been publicly designated.

What does AMBOSS do?

AMBOSS is a Berlin-based medical education and clinical decision-support company founded in 2012 by physicians, including co-founder and co-CEO Benedikt Hochkirchen. Its platform serves more than one million healthcare professionals across more than 180 countries, offering an interactive medical library, a high-yield question bank for licensing and board examinations, and evidence-based clinical decision tools. The company maintains offices in New York, Cologne, and Cagliari in addition to its Berlin headquarters.

AMBOSS valuation & multiples

No verified revenue or EBITDA figures are publicly available for AMBOSS, so EV/Sales and EV/EBITDA multiples cannot be calculated at this time. As comparable benchmarks become available, deal-level valuation analysis will be updated accordingly. For current sector-level transaction multiples in health-care technology and medical education, refer to the Venionaire DealMatrix Health Care sector multiples pages.

Frequently asked questions

How much did AMBOSS raise in its Venture – Series Unknown?

AMBOSS raised EUR 240 million in a venture financing round, announced in KW 13/2025.

What is AMBOSS's valuation?

AMBOSS's valuation was not disclosed in connection with this financing round.

Who invested in AMBOSS?

The round was funded by KIRKBI, Lightrock, and M&G Investments.

When was AMBOSS founded and where is it based?

AMBOSS was founded in 2012 and is headquartered in Berlin, Germany.

Source: original announcement — read the full report.

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